ABSTRACT
We studid the research status and network analysis of health food containing Angelica sinensis in the past 20 years with the methods of data mining. The key information of health food containing Angelica sinensis was obtained in State Food and Drug Administration (SFDA) webpage, using Bibexcel analysis software generated co-occurrence matrix, and using Ucinet 6.0 Net Draw software drawed the social network graph according to the centrality. The data was calculated by SPSS19.0 software analysis. The results showed that 539 health food containing Angelica sinensis were successfully registered in 1997-2016 years, and the annual number of registration was the overall growth trend, there is a certain gap in R & D ability and level of health food containing Angelica sinensis with Angelica health food, brand advantage had not been established in health food containing Angelica sinensis; Health food containing Angelica sinensis form tend to preparation was very obvious such as capsule and oral liquid. The function of health food containing Angelica sinensis mainly concentrated in immune regulation and immune enhancement, removing chloasma, anemia improvment, anti-fatigue, relaxing bowel, improving sleep and anti-aging; and the compatibility of herbs with Angelica sinensis most often were Radix astragali membranacei, Radix rehmanniae, Poria cocos, Radix panacis ginseng, Radix codonopsitis pilosulae, Fructus zizyphi jujubae, Fructus lycii chinesis, Radix rehmanniae, Radix dioscoreae oppositae, Radix albus paeoniae lactiflorae, Colla Corii Asini, Saffron crocus, although compatibility is different, but the quality indexes are similar, the main detection of total polysaccharide and total saponin and total flavonoids. Research and development of health food containing Angelica sinensis should be in the form of regular food and adopt a multi index quality control model in future.
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Objective To study the effects of psychological stress on breast development and abnormal levels of related hormones or their receptors in pregnant rats .Methods Pregnant Wistar rats were randomly divided into control group and ex-perimental group.The experimental group rats were given unpredictable stress stimuli for 15 days, including noise, restraint, re-versal of day and night , swimming in cold water and tail pinch-induced pain.Gross changes of the breast were observed , the di-ameter and height of breast nipple was measured, and breast coefficient was calculated.DNA and RNA levels in the breast tis-sues were determined by optical density measurement.The estrogen (E2), progesterone (P), growth hormone (GH) and prolac-tin (PRL) levels in plasma and breast tissues were determined by radioimmunoassay.Bmax and Kd of estradiol and progesterone receptors were measured by radolig and bindng assay .Morphological changes of the breast tissues were examined by light micros-copy using HE staining.Results Compared with the control group, the nipple diameter and height, breast weight and breast co-efficient were significantly decreased in the experimental group (P<0.05, P<0.01), the levels of DNA, RNA and RNA/DNA ratio in the breast tissue were significantly decreased (P<0.05, P<0.01), the levels of plasma E2, P and GH were significant-ly decreased (P<0.05, P<0.01), the levels of E2 and GH in the breast tissue homogenate were decreased also (P<0.05, P<0.01), the Bmaxs of E2 receptor and P receptor were decreased, but their Kds increased significantly (P<0.05, P<0.01), and the number of lobules and diameter of acini were significantly decreased (P<0.05).Conclusions Psychological stress can lead to breast dysplasia and abnormal levels of estrogen and progesterone and their receptors in pregnant rats .
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Objective To determine the content of ferulic acid, water and extract of Angelica formula particles, provide a reliable method for evaluation and quality control, and compare the quality of Angelica formula particles and Angelica pieces. Methods HPLC method was used with Kromasil C18 column (4.6 mm×250 mm, 5μm), flow rate of 1.0 mL/min, detection wavelength of 316 nm, column temperature at 28 ℃. The mobile phase was acetonitrile-0.05%phosphoric acid in water gradient elution system. Results Ferulic acid showed good linear relationship in the range of 0.033-0.165 μg (r=0.999 8). The average recovery was 98.78% and RSD=0.99%. Angelica formula particles met the quality requirement in the content indicators, the content of ferulic acid was higher than Angelica pieces. Conclusion The HPLC method for determination of ferulic acid in Angelica formula particles is simple and reliable, with good reproducibility, and can be used as the quality control method of Angelica formula particles.
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Objective To build a method to simultaneously determine paeoniflorin, naringin, hesperidin, glycyrrhizic acid in Sinisan by HPLC, provide a reliable method for evaluation and effective quality control, and lay the foundation of material basis for efficacy research for TCM compound Sinisan.Methods The column was Kromasil C18 (4.6 mm×250 mm, 5μm);mobile phase was acetonitrile-0.05% phosphoric acid aqueous solution with gradient elution program;the flow rate was 1.0 mL/min;UV detection was performed at 240 nm;the detector drift tube temperature was set at 30℃.Results The linear ranges of paeoniflorin, naringin, hesperidin and glycyrrhizic acid were 0.00225-0.01125μg (r=0.999 7), 0.00390-0.00195μg (r=0.9997), 0.00198-0.00099μg (r=0.999 5), 0.00262-0.00131μg (r=0.999 7), respectively. The average recoveries of paeoniflorin, hesperidin, naringin and glycyrrhizic acid were 97.72% (RSD=1.02%), 98.45% (RSD=1.52%), 97.74% (RSD=1.63%), 98.34% (RSD=1.78%), respectively.Conclusion The method is accurate, simple, reliable and duplicable, and is available for the quality control of Sinisan.